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Functional expression and germline atransmission of a human chromosome fragment in chimaeric mice

Nature Genetics volume 16, pages 133–143 (1997)Cite this article

Abstract

Human chromosomes or chromosome fragments derived from normal fibroblasts were introduced into mouse embryonic stem (ES) cells via microcell-mediated chromosome transfer (MMCT) and viable chimaeric mice were produced from them. Transferred chromosomes were stably retained, and human genes, including immunoglobulin (Ig) κ, heavy, λ genes, were expressed in proper tissue-specific manner in adult chimaeric tissues. In the case of a human chromosome (hChr.) 2-derived fragment, it was found to be transmitted to the offspring through the germline. Our study demonstrates that MMCT allows for introduction of very large amounts of foreign genetic material into mice. This novel procedure will facilitate the functional analyses of human genomes in vivo.

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Authors and Affiliations

  1. Central Laboratories for Key Technology, Kirin Brewery Co., LTD., 1-13-5, Fukuura, Kanazawa-ku, Yokohama, Kanagawa, 236, Japan

    Kazuma Tomizuka, Hitoshi Yoshida, Kaoru Sato, Atsuko Ohguma & Isao Ishida

  2. Department of Molecular and Cell Genetics, School of Life Sciences, Faculty of Medicine, Tottori University, Nishimachi 86, Yonago, Tottori, 683, Japan

    Hiroshi Uejima, Hiroyuki Kugoh & Mitsuo Oshimura

  3. Laboratory of Molecular Embryology, Department of Bioscience, Kitasato University School of Science, 1-15-1, Kitasato, Sagamihara, Kanagawa, 228, Japan

    Michiko Hayasaka & Kazunori Hanaoka

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  1. Kazuma Tomizuka
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Correspondence to Isao Ishida.

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Tomizuka, K., Yoshida, H., Uejima, H. et al. Functional expression and germline atransmission of a human chromosome fragment in chimaeric mice. Nat Genet 16, 133–143 (1997). https://doi.org/10.1038/ng0697-133

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