Published online 4 August 2010 | Nature 466, 675 (2010) | doi:10.1038/466675a

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Salamander's egg surprise

Algae enjoy symbiotic relationship with embryos.

PUNTA DEL ESTE, URUGUAY

Scientists have stumbled across the first example of a photosynthetic organism living inside a vertebrate's cells. The discovery is a surprise because the adaptive immune systems of vertebrates generally destroy foreign biological material. In this case, however, a symbiotic alga seems to be surviving unchallenged — and might be giving its host a solar-powered metabolic boost.

Algae cohabit with salamander embryos in their eggs &x2014; and inside their cells.Algae cohabit with salamander embryos in their eggs — and inside their cells.T. LEVIN/PHOTOLIBRARY.COM

The embryos of the spotted salamander (Ambystoma maculatum) have long been known to enjoy a mutualistic relationship with the single-celled alga Oophila amblystomatis. The salamanders' viridescent eggs are coloured by algae living in the jelly-like material that surrounds the embryo. The embryos produce nitrogen-rich waste that is useful to the algae, which, in turn, supply the developing embryos with extra oxygen. The algae clearly benefit their salamander hosts: Lynda Goff, a molecular marine biologist at the University of California, Santa Cruz, showed 30 years ago that salamander embryos lacking algae in their surrounding jelly are slower to hatch.

Ryan Kerney of Dalhousie University in Halifax, Nova Scotia, Canada, has now found that these algae also live inside the embryo's cells. Such a close coexistence with a photosynthetic organism has previously been found only in invertebrates, such as corals. Kerney took long-exposure fluorescent images of pre-hatchling salamander embryos, and saw scattered dots in the unstained tissue — an indicator that it might contain chlorophyll. Transmission electron microscopy (TEM) images showed mitochondria in the salamander cells clustering close to the algae. Reporting the discovery on 28 July at the Ninth International Congress of Vertebrate Morphology in Punta del Este, Kerney suggested that the mitochondria might be taking advantage of both oxygen and carbohydrate generated by the alga's photosynthesis.

So when do the algae enter the embryos' cells? A time-lapse video made by Roger Hangarter at Indiana University in Bloomington, and presented by Kerney at the meeting, reveals a fluorescent green flash — an algal bloom — next to each embryo just as its nervous system begins to form. Most research on spotted salamander embryos has focused on earlier periods of development, which might explain why algae have not been seen inside the cells before.

One of Kerney's most curious discoveries suggests that the algae may be a maternal gift. He has found the same algae in the oviducts of adult female spotted salamanders, where the embryo-encompassing jelly sacs first form.

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David Wake, an emeritus professor at the University of California, Berkeley, who watched Kerney's presentation, wonders whether algae could be getting into the reproductive cells. This would "really challenge the dogma" that vertebrates' immune systems ban such close relationships, he says. Both Wake and David Buckley, who studies salamander development at the National Museum of Natural Sciences in Madrid, agree that the work might tell us more about how vertebrate cells learn to identify intruders.

"It makes me wonder if other species of salamander that have known symbiotic relationships with algae also harbour algae inside their cells," adds Daniel Buchholz, a developmental biologist at the University of Cincinnati in Ohio. "I think that if people start looking we may see many more examples." 

For a longer version of this story, see http://go.nature.com/L2DrRP

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  • #60969

    Females and males never even interact for the process. The males drop their spermatophore in the pool of water and the females come along later, collect the sperm to fertilize their eggs, and then lay the eggs. The egg sac isn't quite as big as you see here at first. I think these little guys are close to hatching so the whole thing has grown a bit. In fact, if the water was warmer they'd probably have hatched later that day or the next.

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