Nature Neuroscience 9, 993 - 995 (2006)
Published online: 16 July 2006; | doi:10.1038/nn1742
IGF-1 receptor is essential for the establishment of hippocampal neuronal polarityLucas Sosa, Sebastian Dupraz, Lisandro Laurino, Flavia Bollati, Mariano Bisbal, Alfredo Cáceres, Karl H Pfenninger &
Santiago Quiroga Supplementary Fig. 1 (pdf 324K)
Specificity of the anti-active IGF-1R antibody. Supplementary Fig. 2 (pdf 344K)
Tau did not distribute to a single neurite in  IR3 treated neurons. Supplementary Fig. 3 (pdf 276K)
Transfection with an IGF-1R targeted siRNA silenced the expression of  gc-containing IGF-1R to non-detectable levels and inhibited hippocampal neuron polarization. Supplementary Fig. 4 (pdf 392K)
siRNA-treated neurons showed no significant differences in viability, whereas significant differences were evident in neuronal polarization. Supplementary Fig. 5 (pdf 368K)
siRNA treated neurons expressed near-normal levels of activatable BDNF receptor but were unable to form axons. Supplementary Fig. 6 (pdf 328K)
Neurons expressed insulin receptor (not affected by IGF-1R-targeted siRNA). Supplementary Fig. 7 (pdf 284K)
Co-transfection with a dominant-negative form of cdc-42 did not produce any noticeable effects in the IGF-1R-suppressed neurons. Supplementary Methods (pdf 132K)
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