Nature Neuroscience 9, 599 - 601 (2006)
Published online: 9 April 2006; | doi:10.1038/nn1685
A genetic interaction between the vesicular acetylcholine transporter VAChT/UNC-17 and synaptobrevin/SNB-1 in C. elegansGisela M Sandoval1, Janet S Duerr2, Jonathan Hodgkin3, James B Rand4
& Gary Ruvkun1, 51
Program in Neuroscience, Harvard Medical School, Boston, Massachusetts 02115, USA. 2
Department of Biological Sciences, Ohio University, Athens, Ohio 45701, USA. 3
Genetics Unit, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK. 4
Program in Molecular, Cell and Developmental Biology, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA. 5
Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA.
Correspondence should be addressed to Gary Ruvkun ruvkun@molbio.mgh.harvard.edu Acetylcholine, a major excitatory neurotransmitter in Caenorhabditis elegans, is transported into synaptic vesicles by the vesicular acetylcholine transporter encoded by unc-17. The abnormal behavior of unc-17(e245) mutants, which have a glycine-to-arginine substitution in a transmembrane domain, is markedly improved by a mutant synaptobrevin with an isoleucine-to-aspartate substitution in its transmembrane domain. These results suggest an association of vesicular soluble N-ethylmaleimide-sensitive-factor attachment protein receptor (SNARE) components with vesicular neurotransmitter transporters.
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