2-Deoxy-D-glucose reduces epilepsy progression by NRSF-CtBP–dependent metabolic regulation of chromatin structure

Mireia Garriga-Canut, Barry Schoenike, Romena Qazi, Karen Bergendahl, Timothy J Daley, Rebecca M Pfender, John F Morrison, Jeffrey Ockuly, Carl Stafstrom, Thomas Sutula & Avtar Roopra

Supplementary Fig. 1 (pdf 48K)
Prior to assessing the effects of 2DG on kindling, we sought to determine whether administration of 2DG resulted in gene expression changes indicative of reduced glycolysis in the hippocampus.

Supplementary Fig. 2 (pdf 56K)
Hippocampii from rats treated with saline or 250mg/kg 2DG for 2 weeks were harvested and protein extracted using IP buffer.

Supplementary Fig. 3 (pdf 60K)
The TrkB possesses a functional NRSE. The TrkB NRSE functions in reporter assays. The chromosomal TrkB gene is repressed by NRSF.

Supplementary Fig. 4 (pdf 64K)
Repression of chromosomal NRSF target genes JTC-19 cells is augmented by 2DG.

Supplementary Fig. 5 (pdf 36K)
Wild type MEFs or MEFs heterozygous for both CtBP1 and CtBP2 (CtBP1^-/+2^-/+) or homozygous for both CtBP1 and CtBP2 (CtBP1^-/-2^-/-) grown in the absence or presence of 1mM 2DG were analysed for CHRM4 and HPRT expression by QRT-PCR.

Supplementary Fig. 6 (pdf 28K)
Animal handling and kindling.

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