Nature Neuroscience
- 9, 1247 - 1256 (2006)
Published online: 17 September 2006; | doi:10.1038/nn1775
Asymmetrical  -actin mRNA translation in growth cones mediates attractive turning to netrin-1Kin-Mei Leung, Francisca PG van Horck, Andrew C Lin, Rachel Allison, Nancy Standart & Christine E Holt Supplementary Fig. 1 (pdf 2M)
Netrin-1 induces transport of endogenous Vg1RBP and  -actin mRNA into filopodia. Supplementary Fig. 2 (pdf 3M)
Differential -actin translation in response to attractive and repulsive cues. Supplementary Fig. 3 (pdf 752K)
Model for polarized synthesis of -actin. Supplementary Video 1 (mov 1M)
Movement of Vg1RBP-eGFP granules in retinal growth cones. Retinal explants taken from stage 33/34 embryos injected with Vg1RBP-eGFP mRNA were cultured for 24 hours and analyzed in real time. Images were taken every 12 seconds. Vg1RBP-eGFP granules show bidirectional movement in the axon shaft, growth cone central domain and filopodia. Supplementary Video 2 (mov 600K)
The effect of cytochalasin D on Vg1RBP-eGFP granules movement. Live imaging of Vg1RBP-eGFP positive growth cones. Bath application of cytochalasin D (0.1  M) results in retraction of Vg1RBP-eGFP granules from the growth cone. Images were taken every 12 seconds. Supplementary Video 3 (mov 1M)
Movement of Vg1RBP-eGFP granules into filopodial contact sites. Live imaging of Vg1RBP-eGFP positive growth cones. The arrows indicate anterograde transport of a Vg1RBP-eGFP granule moving to the contact site, followed by a pause and retrograde movement to the base of the filopodium. Images were taken every 12 seconds. Supplementary Video 4 (mov 2M)
Netrin-1 induces transport of Vg1RBP-eGFP granules into filopodia. Vg1RBP-eGFP expressing retinal growth cones were stimulated with netrin-1 after 5 minutes and followed in real time. Images were taken every 12 seconds for 15 minutes. The arrows indicate filopodia in which the increased transport of Vg1RBP-eGFP granules is very prominent. Supplementary Methods (pdf 132K)
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