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Article
Nature Neuroscience  8, 865 - 872 (2005)
Published online: 12 June 2005; | doi:10.1038/nn1479

Neuronal fate determinants of adult olfactory bulb neurogenesis

Michael A Hack1, 6, Armen Saghatelyan2, 6, Antoine de Chevigny2, Alexander Pfeifer3, Ruth Ashery-Padan4, Pierre-Marie Lledo2 & Magdalena Götz1, 5

1  GSF-National Research Center for Environment and Health, Institute for Stem Cell Research, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany.

2  Laboratory of Perception and Memory, Centre National de la Recherche Scientifique, Unité de Recherche Associée 2182, Pasteur Institute, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France.

3  Molecular Pharmacology, Department of Pharmacy, Center for Drug Research, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377 Munich, Germany.

4  Department of Human Genetics and Molecular Medicine, Sackler Faculty of Medicine, Ramat Aviv, Tel Aviv 69987 Israel.

5  Institute of Physiology, University of Munich, Pettenkoferstr. 12, 80336 Munich, Germany.

6  These authors contributed equally to this work.

Correspondence should be addressed to Magdalena Götz magdalena.goetz@gsf.de or Pierre-Marie Lledo pmlledo@pasteur.fr
Adult neurogenesis in mammals is restricted to two small regions, including the olfactory bulb, where GABAergic and dopaminergic interneurons are newly generated throughout the entire lifespan. However, the mechanisms directing them towards a specific neuronal phenotype are not yet understood. Here, we demonstrate the dual role of the transcription factor Pax6 in generating neuronal progenitors and also in directing them towards a dopaminergic periglomerular phenotype in adult mice. We present further evidence that dopaminergic periglomerular neurons originate in a distinct niche, the rostral migratory stream, and are fewer derived from precursors in the zone lining the ventricle. This regionalization of the adult precursor cells is further supported by the restricted expression of the transcription factor Olig2, which specifies transit-amplifying precursor fate and opposes the neurogenic role of Pax6. Together, these data explain both extrinsic and intrinsic mechanisms controlling neuronal identity in adult neurogenesis.

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Nature Neuroscience
ISSN: 1097-6256
EISSN: 1546-1726
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