The function of supramedullary glycine receptors (GlyRs) is still unclear. Using Wistar rat collicular slices, we demonstrate GlyR-mediated inhibition of spike discharge elicited by low glycine (10 M). Searching for the molecular basis of this phenomenon, we identified a new GlyR isoform. GlyR3P185L, a result of cytidine 554 deamination, confers high glycine sensitivity (EC505 M) to neurons and thereby promotes the generation of sustained chloride conductances associated with tonic inhibition. The level of GlyR3-C554U RNA editing is sensitive to experimentally induced brain lesion, inhibition of cytidine deamination by zebularine and inhibition of mRNA transcription by actinomycin D, but not to blockade of protein synthesis by cycloheximide. Conditional regulation of GlyR3P185L is thus likely to be part of a post-transcriptional adaptive mechanism in neurons with enhanced excitability.
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
3P185L, resulting in high agonist potency
M). Searching for the molecular basis of this phenomenon, we identified a new GlyR isoform. GlyR
3P185L, a result of cytidine 554 deamination, confers high glycine sensitivity (EC50 
5 
