High frequency, synchronized bursting drives eye-specific segregation of retinogeniculate projections

Supplementary Fig. 1 (gif 22K)
Correlation index does not change as a function of bin size. Correlation index was computed as previously stated for binwidths from 10 ms to 500 ms.

Supplementary Fig. 2 (jpg 44K)
-galactosidase expression in the developing dLGN. Both Cx36−/− (top row) and Cx36+/− (bottom row) dLGNs reveal a transient expression of -gal throughout development. Scale = 100 µm. Insets: -gal expression is localized to somata within the dLGN. Scale = 5 µm. The existence of Cx36 in the dLGN cannot account for our results since eye−specific segregation was normal in Cx36−/− mice.

Supplementary Fig. 3 (gif 10K)
Cell-attached recording of a single burst from a P10 Cx36−/− RGC indicates spike adaptation occurs in RGCs. Recordings were made from acutely isolated Cx36−/− retinas perfused with oxygenated ACSF and warmed to 32-35° C. The internal electrode solution contained (in mM): 98.3 potassium gluconate, 1.7 KCl, 0.6 EGTA, 5 MgCl2, 2 Na2-ATP, 0.3 GTP, and 40 HEPES, pH 7.25, with KOH. Voltage-clamp cell-attached recordings were made using an Axopatch 200B amplifier and pClamp6 software (Axon Instruments, Foster City, CA).

Supplementary Video 1 (mov 626K)
Time-Lapse Representation of Firing Rates Recorded in Wild-Type and Cx36−/− Retinal Neurons Using a Multielectrode Array For each movie, dots represent the positions of electrodes in the multielectrode array on which discreet units were recorded. The size of each dot in every frame represents the average firing rate recorded over 500 ms on that electrode, larger dots correspond to higher firing rates. The movie plays at 10 frames/s (i.e., five times as fast as real time) and represents five minutes of recording. Only electrodes in which unambiguous units could be isolated from each other are illustrated. If multiple units were recorded on the same electrode, units were randomly eliminated so only one unit was portrayed on each electrode.

Supplementary Video 2 (mov 735K)
Time-Lapse Representation of Firing Rates Recorded in Wild-Type and Cx36−/− Retinal Neurons Using a Multielectrode Array For each movie, dots represent the positions of electrodes in the multielectrode array on which discreet units were recorded. The size of each dot in every frame represents the average firing rate recorded over 500 ms on that electrode, larger dots correspond to higher firing rates. The movie plays at 10 frames/s (i.e., five times as fast as real time) and represents five minutes of recording. Only electrodes in which unambiguous units could be isolated from each other are illustrated. If multiple units were recorded on the same electrode, units were randomly eliminated so only one unit was portrayed on each electrode.

Supplementary Video 3 (mov 685K)
Time-Lapse Representation of Firing Rates Recorded in Wild-Type and Cx36−/− Retinal Neurons Using a Multielectrode Array For each movie, dots represent the positions of electrodes in the multielectrode array on which discreet units were recorded. The size of each dot in every frame represents the average firing rate recorded over 500 ms on that electrode, larger dots correspond to higher firing rates. The movie plays at 10 frames/s (i.e., five times as fast as real time) and represents five minutes of recording. Only electrodes in which unambiguous units could be isolated from each other are illustrated. If multiple units were recorded on the same electrode, units were randomly eliminated so only one unit was portrayed on each electrode.

Supplementary Video 4 (mov 1,267K)
Time-Lapse Representation of Firing Rates Recorded in Wild-Type and Cx36−/− Retinal Neurons Using a Multielectrode Array For each movie, dots represent the positions of electrodes in the multielectrode array on which discreet units were recorded. The size of each dot in every frame represents the average firing rate recorded over 500 ms on that electrode, larger dots correspond to higher firing rates. The movie plays at 10 frames/s (i.e., five times as fast as real time) and represents five minutes of recording. Only electrodes in which unambiguous units could be isolated from each other are illustrated. If multiple units were recorded on the same electrode, units were randomly eliminated so only one unit was portrayed on each electrode.

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