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Brief Communication
Nature Neuroscience  7, 917 - 918 (2004)
Published online: 22 August 2004; | doi:10.1038/nn1303

Lateral organization of endocytic machinery in dendritic spines

Bence Rácz1, Thomas A Blanpied3, Michael D Ehlers3, 4 & Richard J Weinberg1, 2

1  Department of Cell & Developmental Biology, CB #7090, University of North Carolina, Chapel Hill, North Carolina 27599, USA.

2  Department of Neuroscience Center, CB #7090, University of North Carolina, Chapel Hill, North Carolina 27599, USA.

3  Department of Neurobiology, Box 3209, Duke University Medical Center, Durham, North Carolina 27710, USA.

4  Departments of Cell Biology and of Pharmacology and Cancer Biology and Neuroproteomics Laboratory, Box 3209, Duke University Medical Center, Durham, North Carolina 27710, USA.

Correspondence should be addressed to Michael D Ehlers ehlers@neuro.duke.edu
Postsynaptic membrane trafficking plays an important role in synaptic plasticity, but the organization of trafficking machinery within dendritic spines is poorly understood. We use immunocytochemical analysis of rat hippocampal neurons to show that proteins mediating endocytosis are systematically arrayed within dendritic spines, tangential to the synapse. Thus, previously unrecognized lateral domains of the spine organize endocytic protein machinery at sites removed from the postsynaptic density.


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Nature Neuroscience
ISSN: 1097-6256
EISSN: 1546-1726
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