Baz, Par-6 and aPKC are not required for axon or dendrite specification in Drosophila

Supplementary Fig. 1 (jpg 52K)
Par-6 is not detectable in par-6 mutant mushroom body neuroblasts. GFP-marked par-6 mutant clone in the mushroom body of a third instar brain, dotted lines. Neuroblasts, highlighted by Miranda (Mir), have high levels of Par-6 outside the clone and none inside the clone. In addition, aPKC mutant clones have no detectable aPKC protein at this stage⁴.

Supplementary Fig. 2 (jpg 50K)
Baz, Par-6 and aPKC are polarized in mature sensory or CNS neurons. (a) Baz and aPKC localization in ciliated neurons of the embryonic chordotonal organ. 22C10 (green) reveals chordotonal neuron morphology. Baz (red) localized to two puncta; the proximal one is in the dendrite (data not shown). aPKC (red) and Par-6 (not shown) localized in the scolopale support cell that surrounds the sensory dendrite. baz, par-6, and aPKC mutant embryos or larvae show no defects in sensory dendrite morphology, but we cannot be sure all maternal protein is gone at the time of dendrite formation (data not shown). (b) Baz, Par-6, and aPKC polarized localization in third instar larval mushroom body interneurons. Dlg or GFP mark mushroom body architecture. Confocal sections are indicated by the red line (right schematic). Each of the single confocal sections shown contains mushroom body dendrites (D) and proximal axons (A) so that relative protein levels in dendrites and axons can be directly compared. The dendrite region contains synapses, the proximal axons do not. Arrows indicate young axons which extend down the middle of the axon bundle as they develop.

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