Nature Neuroscience7, 1296 - 1298 (2004)
Published online: 14 November 2004; | doi:10.1038/nn1348
Adapter protein 14-3-3 is required for a presynaptic form of LTP in the cerebellum
Fatma Simsek-Duran1, David J Linden2
& György Lonart1
1
Department of Pathology & Anatomy, Eastern Virginia Medical School, Norfolk, Virginia 23501, USA.
2
Department of Neuroscience, Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, Maryland 21205, USA.
Correspondence should be addressed to György Lonart lonartg@evms.edu
Long-term potentiation (LTP) of granule cell−Purkinje cell synapses in the mouse cerebellum requires phosphorylation by protein kinase A of the active-zone protein RIM1 at Ser413. Here, we show that the adapter protein 14-3-3 readily binds phosphorylated Ser413 in RIM1, and that presynaptic transfection with a dominant-negative 14-3-3 mutant, or a RIM1 mutant with enhanced 14-3-3 binding, inhibits LTP. Thus, RIM1 phosphorylation triggers presynaptic LTP in part through recruitment of 14-3-3 to phospho-Ser413−RIM1.
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at Ser413. Here, we show that the adapter protein 14-3-3 readily binds phosphorylated Ser413 in RIM1
mutant, or a RIM1
