GFAP-expressing progenitors are the principal source of constitutive neurogenesis in adult mouse forebrain

Supplementary Fig. 1 (pdf 154K)
Subpopulations of neurons express reporter protein in the olfactory bulbs and dentate gyrus, but not in the striatum. Single channel (a₁-a₂, b₁-b₂, c₁-c₂) and merged images (a₃, b₃, c₃) of the confocal micrographs seen in Figure 5h, i, and k, respectively, showing Gal (green, a₁) or GFP (green, b₁, c₁) and NeuN (blue, a₂, b₂, c₂) double immunofluorescence staining in the striatum (a₁-a₃), olfactory bulb (b₁-b₃), and dentate gyrus (c₁-c₃). (a₁-a₃) In the striatum, NeuN-positive neurons do not express reporter protein (Gal); a Gal-positive cell that does not co-label with NeuN has the appearance of an astrocyte. (b₁-b₃) In the granule cell layer (GCL) of olfactory bulb, a subset of NeuN-positive cells are double labeled with GFP (arrows). (c₁-c₃) In the GCL of the dentate gyrus, a subset of NeuN-positive cells are double labeled with GFP (arrows); a GFP-positive cell that does not co-label with NeuN has the appearance of an astrocyte (arrowhead).

Supplementary Fig. 2 (pdf 142K)
Dividing GFAP-positive/TK-positive cells in the SEZ and SGZ have a bipolar or unipolar morphology. Single channel (a₁-a₃, b₁-b₃) and merged images (a₄, b₄) of the confocal micrographs seen in Figure 6a and b. Triple immunofluorescence staining for BrdU (red), GFAP (green) and TK (blue) identifies triple stained cells in the SEZ (a₁-a₄) and SGZ (b₁-b₄). Triple stained areas where red, green and blue overlap appear white in the overlay images. The cell in (a) exhibits triple staining in much of the cell body. The cell in (b) exhibits white triple staining only in a few regions where GFAP positive filaments extend into the cell body, however, examination of all three channels separately and in overlay shows that the cell is clearly triple labeled.

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