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Journal home Advance online publication Current issue Archive Press releases Supplements Focuses Guide to authors Online submission Permissions For referees Free online issue Contact the journal Subscribe Advertising work@npg naturereprints About this site For librarians   NPG Resources Nature Nature Reviews Neuroscience Nature Cell Biology Nature Medicine Neuroscience Gateway UCSD-Nature Signaling Gateway NPG Subject areas Biotechnology Cancer Chemistry Clinical Medicine Dentistry Development Drug Discovery Earth Sciences Evolution & Ecology Genetics Immunology Materials Science Medical Research Microbiology Molecular Cell Biology Neuroscience Pharmacology Physics Browse all publications Article Nature Neuroscience  6, 869 - 876 (2003) Published online: 29 June 2003; | doi:10.1038/nn1086 New role of 2-glutamate receptors in AMPA receptor trafficking and cerebellar function Hirokazu Hirai1, 2, 6, Thomas Launey1, Sumiko Mikawa1, Takashi Torashima1, 2, Dai Yanagihara3, 4, Tsuyoshi Kasaura3, Akihiro Miyamoto3 & Michisuke Yuzaki2, 5 1  Laboratory for Memory & Learning, RIKEN Brain Science Institute, Saitama 351-0198, Japan. 2  Department of Developmental Neurobiology, St. Jude Children's Research Hospital, 332 North Lauderdale, Memphis, Tennessee 38105, USA. 3  Laboratory for Neurophysiology, Toyohashi University of Technology, Toyohashi 441-8580, Japan. 4  CREST, Japan Science and Technology Corporation (JST), Saitama 332-0012, Japan. 5  Department of Physiology, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku Tokyo 160-8582, Japan. 6  Present address: Kobe University, Graduate School of Medicine and PRESTO, Japan Science and Technology Corporation (JST), 7-5-2, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. Correspondence should be addressed to Hirokazu Hirai hirai@med.kobe-u.ac.jpPrevious gene knockout studies have shown that the orphan glutamate receptor 2 (GluR2) is critically involved in synaptogenesis between parallel fibers and Purkinje cells during development. However, the precise function of GluR2 and whether it is functional in the mature cerebellum remain unclear. To address these issues, we developed an antibody specific for the putative ligand-binding region of GluR2, and application of this antibody to cultured Purkinje cells induced AMPA receptor endocytosis, attenuated synaptic transmission and abrogated long-term depression. Moreover, injection of this antibody into the subarachnoidal supracerebellar space of adult mice caused transient cerebellar dysfunction, such as ataxic gait and poor performance in the rotorod test. These results indicate that GluR2 is involved in AMPA receptor trafficking and cerebellar function in adult mice. MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated REFERENCE AMPA Receptors Nature Encyclopaedia of Life Sciences  See all 5 matches for Reference REVIEWS Beyond parallel fiber LTD: the diversity of synaptic and non-synaptic plasticity in the cerebellum Nature Neuroscience Review Article (01 May 2001)  See all 12 matches for Reviews NEWS AND VIEWS Research Notes Nature Medicine News and Views (01 Jul 2003) Neurobiology: A thorny issue Nature News and Views (07 Aug 2003)  See all 3 matches for News And Views RESEARCH Activation of the TRPC1 cation channel by metabotropic glutamate receptor mGluR1 Nature Letters to Editor (20 Nov 2003)  See all 24 matches for Research  Top Abstract Previous | Next Table of contents Full text Download PDF Send to a friend Save this link Open Innovation Challenges Methods of Modeling Adaptation in Populations Deadline: Dec 30 2009 Reward: $15,000 USD The analysis of adaptation with a population is a frequently encountered computational modeling scen... Optimizing Sub-cellular Localization Tags Deadline: Jan 31 2010 Reward: $20,000 USD The Seeker is looking for methods to optimize sub-cellular localization tags for protein expression.... More Challenges Powered by: nature jobs Leadership Fellowships University of Oxford Oxford United Kingdom Neuroscience Faculty Positions University of Tennessee Health Science Center Memphis, Tennessee, USA More science jobs Post a job for free Figures & Tables Supplementary info Export citation Search buyers guide:   ADVERTISEMENT

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