 | Figure 1
Nature Neuroscience
6, 1292 - 1299 (2003)
Published online: 16 November 2003; | doi:10.1038/nn1157
Non-proliferative effects of lysophosphatidic acid enhance cortical growth and foldingMarcy A Kingsbury, Stevens K Rehen, James J A Contos, Christine M Higgins
& Jerold Chun | | | | Figure 1. Ex vivo culture system simulates in vivo neurogenic parameters.
(a) Cresyl violet−stained sagittal section from an E14 cortical hemisphere cultured for 17 h in control medium. Ctx, cortex; OB, olfactory bulb; V, ventricle. Scale bar, 250  m. (b) Cresyl violet−stained cross-section through a control hemisphere showing the location of labeled S-phase cells (brown) following a 1-h BrdU pulse. CP, cortical plate; IZ, intermediate zone; SVZ, subventricular zone; VZ, ventricular zone. (c) Cross-section showing the migration of labeled S-phase cells to the bottom of the VZ following a 5-h BrdU pulse. (d) Cross-section showing that cells initially labeled in S-phase have migrated into the IZ 17 h after a BrdU pulse. (e−h) Cell cycle phase transition ex vivo is similar to in vivo. (e) After 10 h ex vivo, a 1-h BrdU pulse was given to track the progression of labeled S-phase cells through mitosis. The number of cells double-labeled with anti-BrdU and anti-phospho-H3 (an M-phase marker) was counted at 1, 3, 5 and 7 h after the pulse and expressed as a percentage of the total number of mitotic cells. (f−h) Cross-sections immunolabeled with anti-BrdU (green) and anti-phospho-H3 (red) from control hemispheres showing the location of labeled S-phase and M-phase cells, respectively. Cortices showed very few double-labeled cells (yellow) at 1 h (f), many at 5 h (g) and a decreased percentage at 7 h (h). Scale bars in b−d and f−h, 50 m.
|
| | | |  |
|
