Nature Neuroscience
5, 751 - 759 (2002)
Published online: 1 July 2002; | doi:10.1038/nn883
Rapid recruitment of NMDA receptor transport packets to nascent synapsesPhilip Washbourne, Jennie E. Bennett
& A. Kimberley McAllisterSupplementary Fig. 1. (avi 1.5M)
Movement of an NMDAR transport packet. A 3 d.i.v. cerebral cortical neuron transfected with NR1-DsRed and imaged at 35°C, 24 h after transfection. This time-lapse movie demonstrates movement of an NMDAR transport packet (arrow) in a retrograde direction along a proximal dendrite. NR1-DsRed is in red. Images were acquired every 2 min. A total of 17 frames, corresponding to 32 min of imaging, is compressed to 2 s. Supplementary Fig. 2. (avi 1.3M)
Recruitment of NR1-DsRed to a site of contact with an axon growth cone filopodium. Cortical neurons 3 d.i.v. 'trans' cotransfected with NR1-DsRed (red) and GAP43-EGFP (green) were imaged at 34°C, 16 h after transfection. Growth cone filopodia contacted the NR1-positive dendrite repeatedly, causing recruitment of an NMDAR transport packet to the site of contact. Both the contact and the NMDAR transport packet were then stabilized. Images were acquired every 90 s. A total of 15 frames, corresponding to 21 min of imaging, is compressed to 2 s.
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