Nature Neuroscience
5, 1169 - 1176 (2002)
Published online: 7 October 2002; | doi:10.1038/nn952
A transient receptor potential channel expressed in taste receptor cellsCristian A. Pérez1, 2, 7, Liquan Huang1, 2, 3, 7, Minqing Rong1, 2, 4, J. Ashot Kozak1, 2, 5, Axel K. Preuss2, Hailin Zhang2, 6, Marianna Max2
& Robert F. Margolskee1, 21
Howard Hughes Medical Institute, Mount Sinai School of Medicine, New York University, Box 1677, 1425 Madison Avenue, New York, New York 10029, USA
2
Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York University, Box 1677, 1425 Madison Avenue, New York, New York 10029, USA
3
Present address: Monell Chemical Senses Center, 3500 Market Street, Philadelphia, Pennsylvania 19104, USA
4
Cellular Genomics Inc., 36 Industrial Road, Branford, Connecticut 06405, USA
5
Department of Physiology and Biophysics, J. Irvine Hall, Room 285, University of California, Irvine, California 92697, USA
6
Department of Pharmacology, Hebei Medical University, Shijiazhuang, 050017 Hebei Province, China
7
The first two authors contributed equally to this work.
Correspondence should be addressed to Robert F. Margolskee margolskee@msvax.mssm.eduWe used differential screening of cDNAs from individual taste receptor cells to identify candidate taste transduction elements in mice. Among the differentially expressed clones, one encoded Trpm5, a member of the mammalian family of transient receptor potential (TRP) channels. We found Trpm5 to be expressed in a restricted manner, with particularly high levels in taste tissue. In taste cells, Trpm5 was coexpressed with taste-signaling molecules such as  -gustducin, G 13, phospholipase C- 2 (PLC-2) and inositol 1,4,5-trisphosphate receptor type III (IP3R3). Our heterologous expression studies of Trpm5 indicate that it functions as a cationic channel that is gated when internal calcium stores are depleted. Trpm5 may be responsible for capacitative calcium entry in taste receptor cells that respond to bitter and/or sweet compounds.
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13 colocalizes with gustducin in taste receptor cells and mediates IP3 responses to bitter denatonium
