Nature Neuroscience
3, 1079 - 1084 (2000)
doi:10.1038/80591
Two cis-acting elements in the 3' untranslated region of  -CaMKII
regulate its dendritic targetingYasutake Mori1, 3, Kazunori Imaizumi1, 2, 3, Taiichi Katayama1, 2, 3, Takunari Yoneda1, 3
& Masaya Tohyama1, 31
Department of Anatomy and Neuroscience, Graduate School
of Medicine, Osaka University, 2-2 Yamadaoka Suita,
Osaka 565-0871, Japan
2
Discovery Research Laboratory, Tanabe Seiyaku Corporation
, 3-16-89 Kashima, Yodogawaku, Osaka
532-0031, Japan
3
CREST of Japan Science and Technology Corporation,
Kawaguchi, Saitama 332-0012, Japan
Correspondence should be addressed to Yasutake Mori mori@anat2.med.osaka-u.ac.jpDendritic localization of the  subunit of Ca2+/calmodulin-dependent
protein kinase II (CaMKII) mRNA in CNS neurons requires its 3'
untranslated region (3'UTR). We investigated this targeting mechanism
by identifying two cis-acting elements in the 3'UTR. One is a 30-nucleotide
element that mediated dendritic translocation. A homologous sequence in the
3'UTR of neurogranin, transcripts of which also reside in dendrites,
also funtioned in cis to promote its dendritic transport. Other putative elements
in the CaMKII mRNA inhibit its transport in a resting state. This inhibition
was removed in depolarized neurons, and such activity-dependent derepression
was a primary requirement for their dendritic targeting.
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