Microglia, the immune cells of the brain, can have a beneficial effect in Alzheimer's disease by phagocytosing amyloid-β. Two-photon in vivo imaging of neuron loss in the intact brain of living Alzheimer's disease mice revealed an involvement of microglia in neuron elimination, indicated by locally increased number and migration velocity of microglia around lost neurons. Knockout of the microglial chemokine receptor Cx3cr1, which is critical in neuron-microglia communication, prevented neuron loss.
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- Supplementary Text and Figures (5M)
Supplementary Figures 1–5 and Supplementary Methods
- Supplementary Video 1 (4M)
Example of a z stack acquired by two-photon in vivo imaging. Z stack from 650 μm depth to the surface in a living mouse brain. Neurons are labeled with YFP and microglia with GFP.
- Supplementary Video 2 (2M)
Tracking of microglia migration in vivo. 5-week time-lapse example of microglia migration around a neuron. Colored lines representing the tracks of the microglia are superimposed.
- Supplementary Video 3 (2M)
Screening behavior of microglia with extension and retraction of fine processes. The video consists of z stack projections (40 μm) of fluorescence images recorded with a time interval of 5 min 150 μm below the brain surface.
- Supplementary Video 4 (2M)
Turnover rate (TOR) of microglia. Red/green overlay of subsequent time points to visualize gained (green) and lost (red) as well as stable (yellow) areas of microglial processes.