Technical Report abstract
Nature Neuroscience 12, 947 - 953 (2009)
Published online: 14 June 2009 | doi:10.1038/nn.2345
Twin-spot MARCM to reveal the developmental origin and identity of neurons
Hung-Hsiang Yu1,2, Chun-Hong Chen3, Lei Shi1, Yaling Huang1 & Tzumin Lee1,2
A comprehensive understanding of the brain requires the analysis of individual neurons. We used twin-spot mosaic analysis with repressible cell markers (twin-spot MARCM) to trace cell lineages at high resolution by independently labeling paired sister clones. We determined patterns of neurogenesis and the influences of lineage on neuron-type specification. Notably, neural progenitors were able to yield intermediate precursors that create one, two or more neurons. Furthermore, neurons acquired stereotyped projections according to their temporal position in various brain sublineages. Twin-spot MARCM also permitted birth dating of mutant clones, enabling us to detect a single temporal fate that required chinmo in a sublineage of six Drosophila central complex neurons. In sum, twin-spot MARCM can reveal the developmental origins of neurons and the mechanisms that underlie cell fate.
- Department of Neurobiology, University of Massachusetts, Worcester, Massachusetts, USA.
- Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA.
- Division of Molecular and Genomic Medicine, National Health Research Institutes, Zhunan Town, Miaoli County 350, Taiwan.
Correspondence to: Tzumin Lee1,2 e-mail: tzumin.lee@umassmed.edu
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
NEWS AND VIEWS
New genetic tools for cell lineage analysis in DrosophilaNature Methods News and Views (01 Aug 2009)
RESEARCH
Drosophila Lis1 is required for neuroblast proliferation, dendritic elaboration and axonal transportNature Cell Biology Article (01 Nov 2000)
Genetic mosaic with dual binary transcriptional systems in DrosophilaNature Neuroscience Technical Report (01 May 2006)
Baboon/dSmad2 TGF-β signaling is required during late larval stage for development of adult-specific neuronsThe EMBO Journal Article (08 Feb 2006)
See all 13 matches for Research
