Article abstract
Nature Neuroscience 12, 897 - 904 (2009)
Published online: 31 May 2009 | doi:10.1038/nn.2338
Brain extracellular matrix affects AMPA receptor lateral mobility and short-term synaptic plasticity
Renato Frischknecht1,4, Martin Heine2,3,4, David Perrais2, Constanze I Seidenbecher1, Daniel Choquet2 & Eckart D Gundelfinger1
Abstract
Many synapses in the mature CNS are wrapped by a dense extracellular matrix (ECM). Using single-particle tracking and fluorescence recovery after photobleaching, we found that this net-like ECM formed surface compartments on rat primary neurons that acted as lateral diffusion barriers for AMPA-type glutamate receptors. Enzymatic removal of the ECM increased extrasynaptic receptor diffusion and the exchange of synaptic AMPA receptors. Whole-cell patch-clamp recording revealed an increased paired-pulse ratio as a functional consequence of ECM removal. These results suggest that the surface compartments formed by the ECM hinder lateral diffusion of AMPA receptors and may therefore modulate short-term synaptic plasticity.
- Leibniz Institute for Neurobiology, Magdeburg, Germany.
- Centre National de la Recherche Scientifique, Université de Bordeaux, UMR 5091, Bordeaux, France.
- Present address: Leibniz Institute for Neurobiology, Magdeburg, Germany.
- These authors contributed equally to this work.
Correspondence to: Daniel Choquet2 e-mail: dchoquet@u-bordeaux2.fr
Correspondence to: Eckart D Gundelfinger1 e-mail: gundelfi@ifn-magdeburg.de
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