Article abstract
Nature Neuroscience 12, 568 - 576 (2009)
Published online: 19 April 2009 | doi:10.1038/nn.2318
Myosin-dependent targeting of transmembrane proteins to neuronal dendrites
Tommy L Lewis Jr1, Tianyi Mao2, Karel Svoboda2 & Don B Arnold1
Abstract
The distinct electrical properties of axonal and dendritic membranes are largely a result of specific transport of vesicle-bound membrane proteins to each compartment. How this specificity arises is unclear because kinesin motors that transport vesicles cannot autonomously distinguish dendritically projecting microtubules from those projecting axonally. We hypothesized that interaction with a second motor might enable vesicles containing dendritic proteins to preferentially associate with dendritically projecting microtubules and avoid those that project to the axon. Here we show that in rat cortical neurons, localization of several distinct transmembrane proteins to dendrites is dependent on specific myosin motors and an intact actin network. Moreover, fusion with a myosin-binding domain from Melanophilin targeted Channelrhodopsin-2 specifically to the somatodendritic compartment of neurons in mice in vivo. Together, our results suggest that dendritic transmembrane proteins direct the vesicles in which they are transported to avoid the axonal compartment through interaction with myosin motors.
- Department of Biological Sciences and Program in Molecular and Computational Biology, University of Southern California, Los Angeles, California, USA.
- Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
Correspondence to: Don B Arnold1 e-mail: darnold@usc.edu
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