Technical Report abstract
Nature Neuroscience 12, 229 - 234 (2008)
Published online: 8 December 2008 | doi:10.1038/nn.2247
Bi-stable neural state switches
André Berndt1,5, Ofer Yizhar2,5, Lisa A Gunaydin2,3,5, Peter Hegemann1 & Karl Deisseroth2,4
Abstract
Here we describe bi-stable channelrhodopsins that convert a brief pulse of light into a stable step in membrane potential. These molecularly engineered probes nevertheless retain millisecond-scale temporal precision. Photocurrents can be precisely initiated and terminated with different colors of light, but operate at vastly longer time scales than conventional channelrhodopsins as a result of modification at the C128 position that extends the lifetime of the open state. Because of their enhanced kinetic stability, these step-function tools are also effectively responsive to light at orders of magnitude lower intensity than wild-type channelrhodopsins. These molecules therefore offer important new capabilities for a broad range of in vivo applications.
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Institute of Biology, Experimental Biophysics, Humboldt-University, Invalidenstra
e 42, D-10115 Berlin, Germany. - Department of Bioengineering, Behavioral Sciences, W083 Clark Center, 318 Campus Drive West, Stanford University, Stanford, California 94305, USA.
- Neuroscience Program, Behavioral Sciences, W083 Clark Center, 318 Campus Drive West, Stanford University, Stanford, California 94305, USA.
- Department of Psychiatry and Behavioral Sciences, W083 Clark Center, 318 Campus Drive West, Stanford University, Stanford, California 94305, USA.
- These authors contributed equally to this work.
Correspondence to: Peter Hegemann1 hegemape@rz.hu-berlin.de
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