Supplementary information : Pungent agents from Szechuan peppers excite sensory neurons by inhibiting two-pore potassium channels : Nature Neuroscience

From the following article

Pungent agents from Szechuan peppers excite sensory neurons by inhibiting two-pore potassium channels

Diana M Bautista, Yaron M Sigal, Aaron D Milstein, Jennifer L Garrison, Julie A Zorn, Pamela R Tsuruda, Roger A Nicoll & David Julius

Nature Neuroscience 11, 772 - 779 (2008) Published online: 22 June 2008

doi:10.1038/nn.2143

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Supplementary Text and Figures

Supplementary Figures 1–6 and Supplementary Tables 1 and 2

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Supplementary Movie 1

Both wild type and TRPV1/TRPA1 double mutant mice display aversion to sanshool. The first segment shows a TRPV1/TRPA1 double mutant mouse drinking saccharine-containing water (vehicle). The second segment shows the same mouse drinking saccharine-containing water laced with sanshool (1 mM). Behavior did not differ from that of wild type littermates. The third segment shows the same TRPV1/TRPA1 double mutant mouse drinking saccharine-containing water laced with capsaicin (1 M) and mustard oil (100 M). As expected, no aversive response was observed. In contrast, the final segment shows robust aversion by a wild type littermate after briefly sampling saccharine-containing water laced with capsaicin (1 M) and mustard oil (100 M). Quantification of these behaviors is shown in Fig. 3d.