Supplementary information
From the following article
CNS-derived glia ensheath peripheral nerves and mediate motor root development
Sarah Kucenas, Norio Takada, Hae-Chul Park, Elvin Woodruff, Kendal Broadie & Bruce Appel
Nature Neuroscience 11, 143 - 151 (2008) Published online: 6 January 2008
doi:10.1038/nn2025
Supplementary Text and Figures
Supplementary Tables 1 and 2, and Figures 1–4
Supplementary Video 1
Peripheral nkx2.2a+ cells originate in the CNS. An excerpt from a 24-h time-lapse movie of a Tg(nkx2.2a:megfp) embryo. The images are from a lateral view, focused on the mid-trunk spinal cord. Dorsal is up and anterior is to the left. Sequence shown begins at 40 hpf and ends at approximately 60 hpf. Red circle marks the position where an nkx2.2a+ cell will exit the spinal cord. The migrating cell is outlined in red. Images were collected every 5 min and the movie runs at 3 frames per s. Annotations, including pauses, were added in Openlab (Improvision) before exporting into Quicktime.
Supplementary Video 2
Peripheral nkx2.2a+ cells divide and extend into the periphery. Excerpt from a 24-h time-lapse movie of a Tg(nkx2.2a:megfp) embryo. The images are from a lateral view, focused on the mid-trunk spinal cord. Dorsal is up and anterior is to the left. Sequence shown begins at 52 hpf and ends at approximately 64 hpf. The red ellipses mark peripheral nkx2.2a+ cells. Images were collected every 5 min and the movie runs at 3 frames per s.
Supplementary Video 3
olig2+ motor axons are ensheathed by nkx2.2a+ cells. Excerpt from a 24-h time-lapse sequence of a Tg(nkx2.2a:megfp);Tg(olig2:dsred2) embryo. The images are from a lateral view, focused on the trunk spinal cord. Dorsal is up and anterior is to the left. Sequence shown begins at 52 hpf and ends at approximately 62 hpf. A red circle marks a motor nerve root. Asterisks mark cells dividing into two. Yellow bars throughout the sequence mark the extension of nkx2.2a+ cells along olig2+ axons (red). Images were collected every 10 min and the movie runs at 3 frames per s.
Supplementary Video 4
nkx2.2a+ perineurial glia fail to form sheaths in clsm241 embryos. Excerpt from a 24-h time-lapse sequence of a Tg(nkx2.2a:megfp);clsm241 embryo. The images are from a lateral view, focused on the trunk spinal cord. Dorsal is up and anterior is to the left. Sequence shown begins at 48 hpf and ends at approximately 72 hpf. The red ellipse in the first frame of the sequence marks the position of a developing motor nerve. The first asterisk of the sequence marks the process of a CNS cell (red outline). A second asterisk marks another nkx2.2a+ cell emerging from the spinal cord. Images were collected every 10 min and the movie runs at 3 frames per s.
Supplementary Video 5
nkx2.2a+ perineurial glia fail to form sheaths in clstw11 embryos. Excerpt from a 24-h time-lapse sequence of aTg(nkx2.2a:megfp);clstw11 embryo. The images are from a lateral view, focused on the trunk spinal cord. Dorsal is up and anterior is to the left. Sequence shown begins at 48 hpf and ends at approximately 62 hpf. Red circles mark positions of motor nerves. Asterisks label cells emerging from the CNS. Images were collected every 5 min and the movie runs at 3 frames per s.
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