Articles

  • Article | | open

    Wiskott-Aldrich syndrome protein (WASp) is essential for controlling the cytoskeleton, but its function in innate immunity is unclear. Here the authors show that WASp deficiency is associated with dysregulated septin cage formation, excessive inflammasome activation, elevated immune cell death and reduced bacterial clearance.

    • Pamela P. Lee
    • , Damián Lobato-Márquez
    • , Nayani Pramanik
    • , Andrea Sirianni
    • , Vanessa Daza-Cajigal
    • , Elizabeth Rivers
    • , Alessia Cavazza
    • , Gerben Bouma
    • , Dale Moulding
    • , Kjell Hultenby
    • , Lisa S. Westerberg
    • , Michael Hollinshead
    • , Yu-Lung Lau
    • , Siobhan O. Burns
    • , Serge Mostowy
    • , Mona Bajaj-Elliott
    •  & Adrian J. Thrasher
  • Article | | open

    The source of the Transantarctic Mountains’ high elevation has remained unclear. Here, the authors present data from a 550 km long magnetotelluric geophysical transect showing that uplift is likely to be mechanical via cantilevered flexure along a master boundary fault and not upper mantle or lower crustal thermal mechanisms.

    • Phil Wannamaker
    • , Graham Hill
    • , John Stodt
    • , Virginie Maris
    • , Yasuo Ogawa
    • , Kate Selway
    • , Goran Boren
    • , Edward Bertrand
    • , Daniel Uhlmann
    • , Bridget Ayling
    • , A. Marie Green
    •  & Daniel Feucht
  • Article | | open

    Neutralizing antibodies to oligomannose glycans on HIV Env are difficult to elicit, possibly due to B cell tolerance. Here, Pantophlet et al. synthesize mimetics based on a bacterial oligosaccharide and show that they evoke HIV-neutralizing antibody responses in animals with a human Ig repertoire.

    • Ralph Pantophlet
    • , Nino Trattnig
    • , Sasha Murrell
    • , Naiomi Lu
    • , Dennis Chau
    • , Caitlin Rempel
    • , Ian A. Wilson
    •  & Paul Kosma
  • Article | | open

    Lysosomal distribution is linked to the role of lysosomes in many cellular functions. Here the authors show that the lysosomal protein TMEM55B is regulated by TFEB and recruits JIP4 to the lysosomal surface inducing dynein-dependent transport of lysosomes toward the cell center in response to stress conditions.

    • Rose Willett
    • , José A. Martina
    • , James P. Zewe
    • , Rachel Wills
    • , Gerald R. V. Hammond
    •  & Rosa Puertollano
  • Article | | open

    Leishmaniasis is a parasitic disease transmitted by the bite of infected sand flies. Here the authors describe an atomic resolution cryo-EM structure of the Leishmania ribosome in complex with the recently approved drug paromomycin (PAR) and highlight conserved elements in the drug binding pocket that mediate PAR deleterious effects on the parasite.

    • Moran Shalev-Benami
    • , Yan Zhang
    • , Haim Rozenberg
    • , Yuko Nobe
    • , Masato Taoka
    • , Donna Matzov
    • , Ella Zimmerman
    • , Anat Bashan
    • , Toshiaki Isobe
    • , Charles L. Jaffe
    • , Ada Yonath
    •  & Georgios Skiniotis
  • Article | | open

    The complete maximisation of the entanglement between two complementary blocks of spins due to the dynamics of spin chains remains to be observed. Here, Pitsios et al. simulate such dynamics by propagating single photons in an integrated photonic circuit.

    • Ioannis Pitsios
    • , Leonardo Banchi
    • , Adil S. Rab
    • , Marco Bentivegna
    • , Debora Caprara
    • , Andrea Crespi
    • , Nicolò Spagnolo
    • , Sougato Bose
    • , Paolo Mataloni
    • , Roberto Osellame
    •  & Fabio Sciarrino
  • Article | | open

    Porous organic networks are of great fundamental and technological interest. Here, the authors synthesize a three-dimensional porous organic network with high specific surface area via a solid-state explosive reaction of hexaethynyl triptycene single crystals containing primer molecules.

    • Seo-Yoon Bae
    • , Dongwook Kim
    • , Dongbin Shin
    • , Javeed Mahmood
    • , In-Yup Jeon
    • , Sun-Min Jung
    • , Sun-Hee Shin
    • , Seok-Jin Kim
    • , Noejung Park
    • , Myoung Soo Lah
    •  & Jong-Beom Baek
  • Article | | open

    Using photosensitive caged-compound for femtosecond crystallography at X-ray free electron lasers would allow the structure determination of reaction intermediates. Here the authors demonstrate the feasibility of this approach with a caged NO-compound and present the initial NO-bound intermediate structure of cytochrome P450 nitric oxide reductase.

    • Takehiko Tosha
    • , Takashi Nomura
    • , Takuma Nishida
    • , Naoya Saeki
    • , Kouta Okubayashi
    • , Raika Yamagiwa
    • , Michihiro Sugahara
    • , Takanori Nakane
    • , Keitaro Yamashita
    • , Kunio Hirata
    • , Go Ueno
    • , Tetsunari Kimura
    • , Tamao Hisano
    • , Kazumasa Muramoto
    • , Hitomi Sawai
    • , Hanae Takeda
    • , Eiichi Mizohata
    • , Ayumi Yamashita
    • , Yusuke Kanematsu
    • , Yu Takano
    • , Eriko Nango
    • , Rie Tanaka
    • , Osamu Nureki
    • , Osami Shoji
    • , Yuka Ikemoto
    • , Hironori Murakami
    • , Shigeki Owada
    • , Kensuke Tono
    • , Makina Yabashi
    • , Masaki Yamamoto
    • , Hideo Ago
    • , So Iwata
    • , Hiroshi Sugimoto
    • , Yoshitsugu Shiro
    •  & Minoru Kubo
  • Article | | open

    The evolution of new sex chromosomes potentially generates reproductive isolation. Here, Bracewell et al. combine crossing experiments with population and functional genomics to characterize neo-sex chromosome evolution and incipient speciation in the mountain pine beetle, Dendroctonus ponderosae.

    • Ryan R. Bracewell
    • , Barbara J. Bentz
    • , Brian T. Sullivan
    •  & Jeffrey M. Good
  • Article | | open

    Tumour hypoxia influences both the immune responses and angiogenesis. Here, the authors show that HIF-1α deletion in NK cells impairs NK cytotoxic activity but inhibit tumour growth by decreasing the infiltration of NK cells that express angiostatic soluble VEGFR-1, thus resulting in non-functional angiogenesis.

    • Ewelina Krzywinska
    • , Chahrazade Kantari-Mimoun
    • , Yann Kerdiles
    • , Michal Sobecki
    • , Takayuki Isagawa
    • , Dagmar Gotthardt
    • , Magali Castells
    • , Johannes Haubold
    • , Corinne Millien
    • , Thomas Viel
    • , Bertrand Tavitian
    • , Norihiko Takeda
    • , Joachim Fandrey
    • , Eric Vivier
    • , Veronika Sexl
    •  & Christian Stockmann
  • Article | | open

    Pericytes are essential for the development, maintenance and function of vascular networks. Here, Eilken and colleagues show that expression of the decoy receptor VEGFR1 by pericytes spatially restricts VEGF signalling, thus regulating VEGF-induced endothelial cell sprouting in developing tissues.

    • Hanna M. Eilken
    • , Rodrigo Diéguez-Hurtado
    • , Inga Schmidt
    • , Masanori Nakayama
    • , Hyun-Woo Jeong
    • , Hendrik Arf
    • , Susanne Adams
    • , Napoleone Ferrara
    •  & Ralf H. Adams
  • Article | | open

    Environmental adaptation is generally studied at the genomic level, but it may also be driven by transcriptional processes. Here, the authors investigate variation in gene expression and RNA editing across diverging populations of Drosophila melanogaster from two microclimates.

    • Arielle L. Yablonovitch
    • , Jeremy Fu
    • , Kexin Li
    • , Simpla Mahato
    • , Lin Kang
    • , Eugenia Rashkovetsky
    • , Abraham B. Korol
    • , Hua Tang
    • , Pawel Michalak
    • , Andrew C. Zelhof
    • , Eviatar Nevo
    •  & Jin Billy Li
  • Article | | open

    The chemokine receptor CXCR3-A is expressed in high grade glioma. Here the authors show that CXCR3-A interacts with LPR1, a protein highly expressed in glioma, and this interaction regulates the trafficking of the receptor ultimately impacting on tumour cell migration.

    • Kevin Boyé
    • , Nadège Pujol
    • , Isabel D Alves
    • , Ya-Ping Chen
    • , Thomas Daubon
    • , Yi-Zong Lee
    • , Stephane Dedieu
    • , Marion Constantin
    • , Lorenzo Bello
    • , Marco Rossi
    • , Rolf Bjerkvig
    • , Shih-Che Sue
    • , Andreas Bikfalvi
    •  & Clotilde Billottet
  • Article | | open

    The RNA-binding protein CsrA regulates the expression of hundreds of bacterial genes. Here, Potts et al. use several approaches to assess the contribution of CsrA to global gene expression in E. coli, revealing new binding targets and physiological roles such as in envelope function and iron homeostasis.

    • Anastasia H. Potts
    • , Christopher A. Vakulskas
    • , Archana Pannuri
    • , Helen Yakhnin
    • , Paul Babitzke
    •  & Tony Romeo
  • Article | | open

    Male pattern baldness (MBP) is a complex trait that has genetic associations. Here, Pirastu and colleagues perform a genome-wide association study to show 71 susceptibility loci associated with MBP — 30 of which are novel — and that these loci can explain 38% of heritability.

    • Nicola Pirastu
    • , Peter K. Joshi
    • , Paul S. de Vries
    • , Marilyn C. Cornelis
    • , Paul M. McKeigue
    • , NaNa Keum
    • , Nora Franceschini
    • , Marco Colombo
    • , Edward L. Giovannucci
    • , Athina Spiliopoulou
    • , Lude Franke
    • , Kari E. North
    • , Peter Kraft
    • , Alanna C. Morrison
    • , Tõnu Esko
    •  & James F. Wilson
  • Article | | open

    Cybergenetics aims to monitor and regulate cellular processes in real-time using computer monitoring and feedback of biological readouts. Here the authors use a feedback loop and periodic forcing to maintain cells with a bistable synthetic circuit near its unstable state.

    • Jean-Baptiste Lugagne
    • , Sebastián Sosa Carrillo
    • , Melanie Kirch
    • , Agnes Köhler
    • , Gregory Batt
    •  & Pascal Hersen
  • Article | | open

    The effect of knots on protein stability and folding kinetics is not well understood. Here the authors combine optical tweezer experiments and calculations to experimentally determine the energy cost for knot formation, which indicates that knotted proteins evolved specific folding pathways because knot formation in unfolded chains is unfavorable.

    • Andrés Bustamante
    • , Juan Sotelo-Campos
    • , Daniel G. Guerra
    • , Martin Floor
    • , Christian M. A. Wilson
    • , Carlos Bustamante
    •  & Mauricio Báez
  • Article | | open

    Active chiral fluids are a special case of active matter in which energy is introduced into rotational motion via local application of torque. Here Banerjee et al. develop a hydrodynamic theory of such active fluids and connect it with odd viscosity which was previously considered an abstract concept.

    • Debarghya Banerjee
    • , Anton Souslov
    • , Alexander G. Abanov
    •  & Vincenzo Vitelli
  • Article | | open

    The role of lncRNA is unclear with respect to epithelial-to-mesenchymal transition (EMT), which is linked to ovarian cancer metastasis. Here, the authors show lncRNA DNM3OS expression contributes to ovarian cancer EMT, cell migration/invasion, and correlates with worse overall patient survival.

    • Ramkrishna Mitra
    • , Xi Chen
    • , Evan J. Greenawalt
    • , Ujjwal Maulik
    • , Wei Jiang
    • , Zhongming Zhao
    •  & Christine M. Eischen
  • Article | | open

    Antiviral immune responses involve clustering of plasmacytoid dendritic cells (pDC) in response to endosomal TLR7-mediated sensing of viral RNA. Here the authors show the GTPase Arl8b controls translocation of TLR7+ endosomes to the periphery of the cell via microtubule interactions, thus enabling pDC clustering and type I interferon production.

    • Shin-Ichiroh Saitoh
    • , Fumiko Abe
    • , Atsuo Kanno
    • , Natsuko Tanimura
    • , Yoshiko Mori Saitoh
    • , Ryutaro Fukui
    • , Takuma Shibata
    • , Katsuaki Sato
    • , Takeshi Ichinohe
    • , Mayumi Hayashi
    • , Kazuishi Kubota
    • , Hiroko Kozuka-Hata
    • , Masaaki Oyama
    • , Yorifumi Kikko
    • , Toshiaki Katada
    • , Kenji Kontani
    •  & Kensuke Miyake
  • Article | | open

    CD4+ T cells secreting interleukin-17 (TH17) have diverse functions in modulating autoimmune diseases. Here the authors show via transcriptome analyses that a subset of human TH 17 co-expressing interferon-γ (TH1/17) has a molecular signature similar to “pathogenic” mouse TH 17 but distinct from “non-pathogenic” mouse TH 17.

    • Dan Hu
    • , Samuele Notarbartolo
    • , Tom Croonenborghs
    • , Bonny Patel
    • , Ron Cialic
    • , Tun-Hsiang Yang
    • , Dominik Aschenbrenner
    • , Karin M. Andersson
    • , Marco Gattorno
    • , Minh Pham
    • , Pia Kivisakk
    • , Isabelle V. Pierre
    • , Youjin Lee
    • , Karun Kiani
    • , Maria Bokarewa
    • , Emily Tjon
    • , Nathalie Pochet
    • , Federica Sallusto
    • , Vijay K. Kuchroo
    •  & Howard L. Weiner
  • Article | | open

    Vortex-induced reconnection originates from non-linear vortex flows due to Kelvin-Helmholtz instability in the Earth’s magnetosphere. Here, the authors perform a large-scale kinetic simulation to unveil dynamics of the vortex-induced reconnection and resulting turbulent mixing process.

    • T. K. M. Nakamura
    • , H. Hasegawa
    • , W. Daughton
    • , S. Eriksson
    • , W. Y. Li
    •  & R. Nakamura
  • Article | | open

    The electron-transferring flavoprotein / butyryl-CoA dehydrogenase (EtfAB/Bcd) complex catalyzes the reduction of crotonyl-CoA and ferredoxins by NADH in anaerobic microbes. Here, the authors present the crystal structure of Clostridium difficile EtfAB/Bcd and discuss the bifurcation mechanism for electron flow.

    • Julius K. Demmer
    • , Nilanjan Pal Chowdhury
    • , Thorsten Selmer
    • , Ulrich Ermler
    •  & Wolfgang Buckel