Abstract
We report what is to our knowledge the first natural RNA that regulates gene expression in response to intracellular ATP. Using a biochemical screen, we found that several putative riboswitches bind ATP in vitro. The ydaO motif specifically bound ATP and regulated expression of endogenous and reporter genes in response to ATP concentrations in Bacillus subtilis. This discovery demonstrates a role for RNAs in regulating gene expression in response to energy balance in bacteria.
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Acknowledgements
We acknowledge J. Viladoms for thoughtful conversations and critical reading of this manuscript. We thank the groups of E. Winzeler and P. Sun for access to qPCR instruments and the Skaggs Institute for Chemical Biology for graduate fellowship support of P.Y. Watson.
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P.Y.W. and M.J.F. conceived of and designed experiments, analyzed the data and prepared the manuscript. P.Y.W. conducted the experiments.
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Watson, P., Fedor, M. The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis. Nat Chem Biol 8, 963–965 (2012). https://doi.org/10.1038/nchembio.1095
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DOI: https://doi.org/10.1038/nchembio.1095
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