Brief Communication abstract
Nature Chemical Biology 5, 403 - 405 (2009)
Published online: 10 May 2009 | doi:10.1038/nchembio.171
Brownian search-and-catch mechanism for myosin-VI steps
Mitsuhiro Iwaki1,2, Atsuko H Iwane1,2, Tetsuya Shimokawa2, Roger Cooke3 & Toshio Yanagida1,2
The cargo transporter myosin-VI processively walks along actin filaments using its two heads. Here we use single-molecule nanometry to show that the strong binding by myosin heads to actin is greatly accelerated (
30-fold) when backward strain is applied to weakly bound heads during the actin search. We propose that the myosin head searches for the forward actin target by Brownian motion and catches the actin in a strain-dependent manner.
- Graduate School of Medicine, Osaka University, Osaka, Japan.
- Soft Biosystem Group, Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan.
- Department of Biochemistry and Biophysics and Cardiovascular Research Institute, University of California, San Francisco, California, USA.
Correspondence to: Toshio Yanagida1,2 e-mail: yanagida@phys1.med.osaka-u.ac.jp
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