Brief Communication abstract

Nature Chemical Biology 4, 405 - 407 (2008)
Published online: 18 May 2008 | Corrected online: 29 May 2008 | doi:10.1038/nchembio.91

Disparate proteome reactivity profiles of carbon electrophiles

Eranthie Weerapana1, Gabriel M Simon1 & Benjamin F Cravatt1

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Insights into the proteome reactivity of electrophiles are crucial for designing activity-based probes for enzymes lacking cognate affinity labels. Here, we show that different classes of carbon electrophiles exhibit markedly distinct amino acid labeling profiles in proteomes, ranging from selective reactivity with cysteine to adducts with several amino acids. These data specify electrophilic chemotypes with restricted and permissive reactivity profiles to guide the design of next-generation functional proteomics probes.

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  1. The Skaggs Institute for Chemical Biology and Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

Correspondence to: Benjamin F Cravatt1 e-mail: cravatt@scripps.edu

* In the version of this article initially published online, the two labels in Figure 2c that read 'CLIC4(C35C)' are incorrect. The correct labels should read 'CLIC4(C35S)'. The error has been corrected for all versions of the article.

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