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Brief Communication

Nature Chemical Biology 4, 599–601 (1 October 2008) | doi:10.1038/nchembio.110

Mechanism of CuA assembly

Luciano A Abriata , Lucia Banci , Ivano Bertini , Simone Ciofi-Baffoni , Petros Gkazonis , Georgios A Spyroulias , Alejandro J Vila & Shenlin Wang

Copper is essential for proper functioning of cytochrome c oxidases, and therefore for cellular respiration in eukaryotes and many bacteria. Here we show that a new periplasmic protein (PCuAC) selectively inserts Cu(I) ions into subunit II of Thermus thermophilus ba3 oxidase to generate a native CuA site.