Figure 1 - HPLC analysis at 254 nm of organic extracts from different reconstituted enc PKS enzyme sets.

From the following article

Enzymatic total synthesis of enterocin polyketides

Qian Cheng, Longkuan Xiang, Miho Izumikawa, Dario Meluzzi & Bradley S Moore

Nature Chemical Biology 3, 557 - 558 (2007) Published online: 12 August 2007

doi:10.1038/nchembio.2007.22

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(a) EncA/B and EncC. (b) EncA/B and EncC plus benzoic acid. (c) EncA/B, EncC, EncD and EncN plus benzoic acid, ATP/Mg2+ and NADPH. (d) EncA/B, EncC, EncD, EncN and EncM plus benzoic acid, ATP/Mg2+ and NADPH. (e) EncA/B, EncC, EncD, EncN, EncM and EncK plus benzoic acid, ATP/Mg2+, NADPH and SAM. (f) EncA/B, EncC, EncD, EncN, EncM, EncK and EncR plus benzoic acid, ATP/Mg2+, NADPH, SAM, ferredoxin, ferredoxin-NADP+ reductase and catalase. (g) Authentic standards used for product analysis. All assays were performed in Tris-HCl buffer containing SgFabD and malonyl-CoA.

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