Supplementary information

From the following article

Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator

Oded Tour, Stephen R Adams, Rex A Kerr, Rene M Meijer, Terrence J Sejnowski, Richard W Tsien & Roger Y Tsien

Nature Chemical Biology 3, 423 - 431 (2007) Published online: 17 June 2007

doi:10.1038/nchembio.2007.4

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Supplementary Fig. 1

Tetracysteine-tagged L-type calcium channel imaged using TIRF-M.

Supplementary Fig. 2

Patch clamp stimulus waveforms.

Supplementary Fig. 3

Representative ensemble whole-cell currents exhibit similar amplitudes and kinetics, regardless of whether cells had untagged alpha1C channels, tagged 4N-GFP-alpha1C channels without CaGF labeling, or tagged 4N-GFP-alpha1C channels labeled by CaGF.

Supplementary Video 1

CaGF reports the spread of calcium.

Supplementary Video 2

Spatial and temporal display of Ca2+ transients triggered by patch clamp activation of L-type calcium channels.

Supplementary Video 3

A patch pipette was filled with 15 mM Ca2+ that diffused into the cell on the right following the break of the cell membrane to establish a whole-cell configuration.

Supplementary Video 4

Spatial and temporal display of four Ca2+ transients triggered by patch clamp activation of L-type calcium channels.

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