Supplementary information
From the following article
Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator
Oded Tour, Stephen R Adams, Rex A Kerr, Rene M Meijer, Terrence J Sejnowski, Richard W Tsien & Roger Y Tsien
Nature Chemical Biology 3, 423 - 431 (2007) Published online: 17 June 2007
doi:10.1038/nchembio.2007.4
Supplementary Fig. 1
Tetracysteine-tagged L-type calcium channel imaged using TIRF-M.
Supplementary Fig. 3
Representative ensemble whole-cell currents exhibit similar amplitudes and kinetics, regardless of whether cells had untagged
1C channels, tagged 4N-GFP-
1C channels without CaGF labeling, or tagged 4N-GFP-
1C channels labeled by CaGF.
Supplementary Video 1
CaGF reports the spread of calcium.
Supplementary Video 2
Spatial and temporal display of Ca2+ transients triggered by patch clamp activation of L-type calcium channels.
Supplementary Video 3
A patch pipette was filled with 15 mM Ca2+ that diffused into the cell on the right following the break of the cell membrane to establish a whole-cell configuration.
Supplementary Video 4
Spatial and temporal display of four Ca2+ transients triggered by patch clamp activation of L-type calcium channels.
Supplementary Methods
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