Supplementary information : Introducing genetically encoded aldehydes into proteins : Nature Chemical Biology

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Introducing genetically encoded aldehydes into proteins

Isaac S Carrico, Brian L Carlson & Carolyn R Bertozzi

Nature Chemical Biology 3, 321 - 322 (2007) Published online: 22 April 2007

doi:10.1038/nchembio878

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Supplementary Fig. 1

Mass spectra confirming the presence of fGly in a tryptic peptide from ald₁₃-Stf0.

Supplementary Fig. 1 - Download PDF file (223KB)

Supplementary Fig. 2

Quantitation of the conversion of cysteine to formylglycine using mass spectrometry.

Supplementary Fig. 2 - Download PDF file (157KB)

Supplementary Fig. 3

Quantitation of the conversion of cysteine to formylglycine using Alexa Fluor 647 C5-aminooxyacetamide labeling.

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Supplementary Fig. 4

PEGylation of ald₆-MBP with 2 kDa and 5 kDa aminooxy-PEG.

Supplementary Fig. 4 - Download PDF file (185KB)

Supplementary Table 1

Oligonucleotides used in this study.

Supplementary Table 1 - Download PDF file (275KB)

Supplementary Methods

Supplementary Methods - Download PDF file (286KB)

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Supplementary information