Figure 2 - O-GlcNAc glycosylation is reversible in cultured cortical neurons.
From the following article
Probing the dynamics of O-GlcNAc glycosylation in the brain using quantitative proteomics
Nelly Khidekel, Scott B Ficarro, Peter M Clark, Marian C Bryan, Danielle L Swaney, Jessica E Rexach, Yi E Sun, Joshua J Coon, Eric C Peters & Linda C Hsieh-Wilson
Nature Chemical Biology 3, 339 - 348 (2007) Published online: 13 May 2007
doi:10.1038/nchembio881
(a) Treatment of cortical neurons with the OGA inhibitor PUGNAc for 12 h enhanced overall O-GlcNAc glycosylation levels in both nuclear and cytoplasmic fractions, as measured by immunoblotting with an anti-O-GlcNAc antibody. (b–d) Peptide mass spectra of three proteins showing distinct activation profiles. O-GlcNAc glycosylation of the peptide in b was upregulated in response to PUGNAc treatment, whereas the glycosylation level was unchanged for the peptide in c and was downregulated for the peptide in d.
