Figure 3 - Temporal effects of neuromodulators on NPC viability and apoptotic response.
From the following article
Chemical genetics reveals a complex functional ground state of neural stem cells
Phedias Diamandis, Jan Wildenhain, Ian D Clarke, Adrian G Sacher, Jeremy Graham, David S Bellows, Erick K M Ling, Ryan J Ward, Leanne G Jamieson, Mike Tyers & Peter B Dirks
Nature Chemical Biology 3, 268 - 273 (2007) Published online: 8 April 2007
doi:10.1038/nchembio873
(a) Proliferation dynamics of PAPP-, ifenprodil- and etoposide-treated NPCs. (b) Normalized caspase-3 and caspase-7 activity in NPCs after 12 h and 48 h of drug treatment. Asterisk indicates a significant change (two-tailed t-test) from the corresponding vehicle-treated data point. (c) Corresponding MTT values taken at 12 h and 2 d for the caspase-3 and caspase-7 experiments shown in b. All values represent the mean and s.d. of one representative experiment (from three independent trials) of NPCs treated with PAPP (1 
M), ifenprodil (3 M), etoposide (3 M) or vehicle. (d) Flow cytometric analysis of the neural precursor marker nestin in NPCs after 2 d of treatment with ifenprodil (5 M) or vehicle. Representative histograms of vehicle-treated (20% nestin negative) and ifenprodil-treated (63% nestin negative) cells compared with the isotype control (100% nestin negative) are shown from two independent experiments.
