Figure 1 - HTS of NPCs.
From the following article
Chemical genetics reveals a complex functional ground state of neural stem cells
Phedias Diamandis, Jan Wildenhain, Ian D Clarke, Adrian G Sacher, Jeremy Graham, David S Bellows, Erick K M Ling, Ryan J Ward, Leanne G Jamieson, Mike Tyers & Peter B Dirks
Nature Chemical Biology 3, 268 - 273 (2007) Published online: 8 April 2007
doi:10.1038/nchembio873
(a) Neurospheres are derived from self-renewing multipotent NSCs and contain a heterogeneous mixture of stem cells, progenitor cells, and a very small number of differentiated cells. (b) Scatter plot of all 1,267 compounds of the LOPAC library screened against NPCs. 160 compounds (
) were identified as inhibitors of neurosphere proliferation (P < 0.01), 19 compounds (
) were identified as activators (P < 0.01) and the remaining agents (
) screened did not have any significant effects on proliferation (P > 0.01). (c) Examples of phenotypic variation observed in response to particular agents. Scale bars, 250 
m. HTS, high-throughput screen.
