Letter abstract


Nature Chemical Biology 3, 263 - 267 (2007)
Published online: 1 April 2007 | Corrected online: 3 May 2007 | doi:10.1038/nchembio871



There is a Corrigendum (June 2007) associated with this Letter.

Molecular imaging of hydrogen peroxide produced for cell signaling

Evan W Miller1, Orapim Tulyathan2, Ehud Y Isacoff2 & Christopher J Chang1

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Hydrogen peroxide (H2O2) is emerging as a newly recognized messenger in cellular signal transduction1, 2. However, a substantial challenge in elucidating its diverse roles in complex biological environments is the lack of methods for probing this reactive oxygen metabolite in living systems with molecular specificity. Here we report the synthesis and application of Peroxy Green 1 (PG1) and Peroxy Crimson 1 (PC1), two new fluorescent probes that show high selectivity for H2O2 and are capable of visualizing endogenous H2O2 produced in living cells by growth factor stimulation, including the first direct imaging of peroxide produced for brain cell signaling. The combined features of reactive oxygen species selectivity, sensitivity to signaling levels of H2O2, and live-cell compatibility presage many new opportunities for PG1, PC1 and related synthetic reagents for exploring the physiological roles of H2O2 in living systems with molecular imaging.

*Note: In the version of this article initially published, the second author's last name is misspelled. The author's name should read Orapim Tulyathan. The error has been corrected in the HTML and PDF versions of the article.

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  1. Department of Chemistry, University of California, 532A Latimer Hall, Berkeley, California 94720, USA.
  2. Department of Molecular and Cell Biology, University of California, Valley Life Sciences Addition, Berkeley, California 94720, USA.

Correspondence to: Christopher J Chang1 e-mail: chrischang@berkeley.edu

* changed from Tulyanthan to Tulyathan, first n removed

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