Supplementary information
From the following article
TRPM8 voltage sensor mutants reveal a mechanism for integrating thermal and chemical stimuli
Thomas Voets, Grzegorz Owsianik, Annelies Janssens, Karel Talavera & Bernd Nilius
Nature Chemical Biology 3, 174 - 182 (2007) Published online: 11 February 2007
doi:10.1038/nchembio862
Supplementary Fig. 1
Examples of the method used to determine Popen.
Supplementary Fig. 2
Fits of two published models describing the temperature dependence of TRPM8 to experimental data.
Supplementary Fig. 3
[3H]menthol displacement experiments on nontransfected HEK 293 cells and on cells expressing TRPM2, WT TRPM8 and TRPM8 mutants.
Supplementary Fig. 4
Plasma membrane expression and current densities for different TRPM8-TRPM5 chimeras.
Supplementary Fig. 5
Additional predictions of the MWC model.
Supplementary Table 1
Parameters that describe the expression level, voltage dependence, temperature dependence and menthol sensitivity of WT and mutant TRPM8.
Supplementary Methods
Procedure for determination of activation curves, comparison of two models describing the temperature dependence of TRPM8, and mathematical description of the MWC and HH models.
DOWNLOAD BROWSER PLUGINS AND OTHER APPLICATIONS
Movie files
PDF documents
Text documents
PostScript documents
Flash movies
Audio files
- Apple iTunes (PC or Mac)
- QuickTime Player (PC or Mac)
- Realplayer (PC or Mac)
- Windows Media player (PC only)
