Supplementary information
From the following article
Vitamin B6 conjugation to nuclear corepressor RIP140 and its role in gene regulation
M D Mostaqul Huq, Nien-Pei Tsai, Ya-Ping Lin, LeeAnn Higgins & Li-Na Wei
Nature Chemical Biology 3, 161 - 165 (2007) Published online: 4 February 2007
doi:10.1038/nchembio861
Supplementary Fig. 1
Chemistry of PLP conjugation to lysine and the proposed MS fragmentation.
Supplementary Fig. 2
The expanded MS/MS spectra (225-240 m/z) of the precursor ions of RIP140 peptide (608-630 aa) from insect cells.
Supplementary Fig. 3
Mapping of PLP modification at Lys-613.
Supplementary Fig. 4
The expanded MS/MS spectra (225-240 m/z) of the precursor ions from RIP140 peptide (606-630 aa).
Supplementary Fig. 5
Identification of PLP modification site at Lys613 on RIP140 in vitro by LC-ESI-MS/MS analysis.
Supplementary Fig. 6
ESI-MS/MS of analysis of in vitro PLP modified synthetic peptide.
Supplementary Fig. 7
PLP conjugation of RIP140 and its biological impacts.
Supplementary Methods
Expression, purification and mass spectrometry of RIP140 and ESI-MS/MS analysis of in vitro PLP-conjugated synthetic peptide.
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