Supplementary information
From the following article
A clickable inhibitor reveals context-dependent autoactivation of p90 RSK
Michael S Cohen, Haralambos Hadjivassiliou & Jack Taunton
Nature Chemical Biology 3, 156 - 160 (2007) Published online: 28 January 2007
doi:10.1038/nchembio859
Supplementary Fig. 1
Inhibition of PMA-stimulated RSK Ser386 phosphorylation by fmk.
Supplementary Fig. 2
fmk-pa covalently modifies RSK1 and RSK2 in intact cells as determined by click chemistry.
Supplementary Fig. 3
Covalent modification of RSK by fmk-pa is unaffected by serum in the incubation medium, whereas covalent modification by fmk-BODIPY is significantly decreased.
Supplementary Fig. 4
fmk-pa does not inhibit S6K phosphorylation at Thr389, an essential activation site.
Supplementary Fig. 5
Inhibition of rpS6 Ser235/236 phosphorylation by rapamycin and fmk-pa.
Supplementary Table 1
Half-maximal inhibitory concentrations (IC50s) for fmk, fmk-BODIPY and fmk-pa against the kinase activity of RSK2 CTD.
Supplementary Methods
Synthetic schemes and procedures.
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