Brief Communication abstract
Nature Chemical Biology 3, 707 - 708 (2007)
Published online: 23 September 2007 | doi:10.1038/nchembio.2007.31
Sortagging: a versatile method for protein labeling
Maximilian W Popp1,2, John M Antos1, Gijsbert M Grotenbreg1, Eric Spooner1 & Hidde L Ploegh1,2
Genetically encoded reporter constructs that yield fluorescently labeled fusion proteins are a powerful tool for observing cell biological phenomena, but they have limitations. Sortagging (sortase-mediated transpeptidation) is a versatile chemoenzymatic system for site-specific labeling of proteins with small (<2 kDa) probes. Sortagging combines the precision of a genetically encoded tag with the specificity of an enzymatic reaction and the ease and chemical versatility of peptide synthesis. Here we apply this technique to proteins in vitro and on the surface of living cells.
- Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA.
- Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02142, USA.
Correspondence to: Hidde L Ploegh1,2 e-mail: ploegh@wi.mit.edu
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