Supplementary information
From the following article
Michael B Austin, Tamao Saito, Marianne E Bowman, Stephen Haydock, Atsushi Kato, Bradley S Moore, Robert R Kay & Joseph P Noel
Nature Chemical Biology 2, 494 - 502 (2006) Published online: 13 August 2006
doi:10.1038/nchembio811
Supplementary Fig. 1
In metazoan type 1 FASs and related type 1 PKSs, a C-terminal TE domain catalyzes the hydrolytic release of enzymatic products from the prosthetic Ppant arm of the adjacent ACP domain.
Supplementary Fig. 2
Acyl-thioester starter specificities of steely C-terminal type 3 PKS domains in comparison to alfalfa CHS.
Supplementary Fig. 3
LC-MS-MS analysis of all hexanoyl-primed products of in vitro enzyme assays with malonyl-CoA.
Supplementary Fig. 4
LC-MS-MS analysis of all butanoyl-primed products of in vitro enzyme assays with malonyl-CoA.
Supplementary Fig. 5
Transformed clones were screened for disruption of the Steely1 (stlA) and Steely2 (stlB) loci by genomic PCR using primers.
Supplementary Table 1
Data collection and refinement statistics.
Supplementary Methods
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