Supplementary information
From the following article
Total biosynthesis of antitumor nonribosomal peptides in Escherichia coli
Kenji Watanabe, Kinya Hotta, Alex P Praseuth, Kento Koketsu, Akira Migita, Christopher N Boddy, Clay C C Wang, Hiroki Oguri & Hideaki Oikawa
Nature Chemical Biology 2, 423 - 428 (2006) Published online: 25 June 2006
doi:10.1038/nchembio803
Supplementary Fig. 1
SDS-PAGE analysis of the purified E. coli-produced echinomycin biosynthetic proteins.
Supplementary Fig. 2
Maps of the plasmid-borne echinomycin biosynthetic gene cluster.
Supplementary Fig. 3
Assays for checking the maintenance and stability of the plasmids carrying the echinomycin biosynthetic cluster in E. coli DH5
.
Supplementary Fig. 4
Chemical characteristic spectra of the authentic reference for echinomycin.
Supplementary Fig. 5
Chemical characteristic spectra of the authentic reference for triostin A.
Supplementary Methods
Methods used for echinomycin biosynthetic cluster isolation and a table of the sequences of the primers used in this manuscript.
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