Figure 2 - Biological activities.
From the following article
Site-specific PEGylation of native disulfide bonds in therapeutic proteins
Sunil Shaunak, Antony Godwin, Ji-Won Choi, Sibu Balan, Elisa Pedone, Damotharan Vijayarangam, Sibylle Heidelberger, Ian Teo, Mire Zloh & Steve Brocchini
Nature Chemical Biology 2, 312 - 313 (2006) Published online: 23 April 2006
doi:10.1038/nchembio786
(a) Antiviral activity in A549 cells infected with EMC virus (n = 6). (b) 2',5'-OAS mRNA synthesis in Molt-4 cells (n = 3). IFN (gray); unreacted IFN recovered after SEC-HPLC (red); non-PEGylated three-carbon disulfide-bridged IFN (green); three-carbon disulfide single-bridged 10 kDa PEG-IFN (orange); three-carbon disulfide single-bridged 20 kDa PEG-IFN (blue). (c) Immunoblot with an antibody to Fab. Mw markers (left) are in kDa. Lanes: (1) Fab; (2) reduced Fab; (3) three-carbon disulfide single-bridged 20 kDa PEG-Fab. (d) Inhibition of HIV-1 entry into human C8166 (T-lymphoblastoid) cells as determined by real-time PCR for RU5, the first DNA transcript of HIV-1 to be synthesized after viral entry (n = 3). Data presented as mean 
s.e.m.
