Supplementary information
From the following article
Proteomic profiling of metalloprotease activities with cocktails of active-site probes
Stephan A Sieber, Sherry Niessen, Heather S Hoover & Benjamin F Cravatt
Nature Chemical Biology 2, 274 - 281 (2006) Published online: 26 March 2006
doi:10.1038/nchembio781
Supplementary Fig. 1
Synthesis, structures and characterization of HxBPyne probe library.
Supplementary Fig. 2
Representative 1D gels showing the proteome reactivity profiles of members of the HxBPyne library.
Supplementary Fig. 3
Labeling of native and recombinant MPs by HxBPyne probes.
Supplementary Fig. 5
Expression levels of MPs in human melanoma cell lines.
Supplementary Fig. 6
Examples of MPs that were found at equivalent levels in HxBPyne-treated proteomes and control proteomes treated either with excess HxBPane probes or without any probe.
Supplementary Table 1
Complete list of specifically labeled MP targets identified by the optimal HxBPyne probe set and ABPP-MudPIT.
Supplementary Methods
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