In This Issue - pv
doi:10.1038/nchembio0206-v
Table of contents
February 2006, Volume 2 No 2 pp53-110
About the coverTop of page
Editorial
Molecular cross-fertilization - p53
doi:10.1038/nchembio0206-53
Full Text - Molecular cross-fertilization | PDF (80 KB) - Molecular cross-fertilization
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Commentary
Challenges facing the biologist doing chemical genetics - pp55 - 58
Jon Soderholm, Mike Uehara-Bingen, Karsten Weis & Rebecca Heald
doi:10.1038/nchembio0206-55
The goal of high-throughput screening (HTS) from the perspective of the biologist is to identify a highly specific small molecule that can be used to inhibit a protein in its normal biological context. Although several useful small molecules have been identified with HTS, there are many challenges to be considered when contemplating a screen, especially by those unfamiliar with chemical biology.
Full Text - Challenges facing the biologist doing chemical genetics | PDF (179 KB) - Challenges facing the biologist doing chemical genetics
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Meeting Report
Symbiosis in chemistry and biology - pp59 - 62
Paul V Murphy & Peter J Rutledge
doi:10.1038/nchembio0206-59
What do organic synthesis, mechanistic enzymology, structural biology and glycoscience have in common? They all span chemistry and biology, benefit from and contribute to multidisciplinary approaches, and were up for discussion at a recent symposium in Dublin.
Full Text - Symbiosis in chemistry and biology | PDF (244 KB) - Symbiosis in chemistry and biology
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News and Views
Inhibitors of Bcr-abl... breaking new ground again - pp63 - 64
Jeffrey F Ohren & Judith S Sebolt-Leopold
doi:10.1038/nchembio0206-63
The report of a new class of potent and highly selective inhibitors of Bcr-abl highlights the potential for a truly leukemia-specific drug with no expected off-target activity. GNF-2, as a representative compound of this class, may have inspired the identification of a region of Bcr-abl that is amenable to drug design, just as imatinib did almost a decade ago.
Full Text - Inhibitors of Bcr-abl... breaking new ground again | PDF (185 KB) - Inhibitors of Bcr-abl... breaking new ground again
See also: Article by Adrián et al.
Getting under wraps: alkylating DNA in the nucleosome - p64
Kent S Gates
doi:10.1038/nchembio0206-64
DNA in the nucleus of mammalian cells is extensively associated with proteins. Potential anticancer agents can access, recognize and alkylate nucleosomal DNA, even at sites that seem completely occluded by association with histone proteins.
Full Text - Getting under wraps: alkylating DNA in the nucleosome | PDF (988 KB) - Getting under wraps: alkylating DNA in the nucleosome
See also: Letter by Trzupek et al.
Michaelis-Menten is dead, long live Michaelis-Menten! - pp66 - 67
Nils G Walter
doi:10.1038/nchembio0206-66
Modern single-molecule tools, when applied to enzymes, challenge fundamental concepts of catalysis by uncovering mechanistic pathways, intermediates and heterogeneities hidden in the ensemble average. It is thus reassuring that the Michaelis-Menten formalism, a pillar of enzymology, is upheld, if reinterpreted, even when visualizing single turnover events with a microscope focus.
Full Text - Michaelis-Menten is dead, long live Michaelis-Menten! | PDF (157 KB) - Michaelis-Menten is dead, long live Michaelis-Menten!
See also: Article by English et al.
Sweet insights into learning and memory - pp67 - 68
Gerald W Hart
doi:10.1038/nchembio0206-67
Studies have linked the Fuc
(1-2)Gal modification of neuronal glycoproteins to cognitive processes such as learning and memory. The identification of synapsins Ia and Ib as the main Fuc(1-2)Gal-bearing neuronal proteins not only suggests previously unknown molecular mechanisms of neuronal plasticity but also indicates the existence of previously unknown glycosylation pathways in neurons.
Full Text - Sweet insights into learning and memory | PDF (213 KB) - Sweet insights into learning and memory
Research Highlights - p69
doi:10.1038/nchembio0206-69
Full Text - Research Highlights | PDF (129 KB) - Research Highlights
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Review
Rethinking 'secondary' metabolism: physiological roles for phenazine antibiotics - pp71 - 78
Alexa Price-Whelan, Lars E P Dietrich & Dianne K Newman
doi:10.1038/nchembio764
Abstract - Rethinking 'secondary' metabolism: physiological roles for phenazine antibiotics | Full Text - Rethinking 'secondary' metabolism: physiological roles for phenazine antibiotics | PDF (344 KB) - Rethinking 'secondary' metabolism: physiological roles for phenazine antibiotics
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Letters
Alkylation of duplex DNA in nucleosome core particles by duocarmycin SA and yatakemycin - pp79 - 82
John D Trzupek, Joel M Gottesfeld & Dale L Boger
doi:10.1038/nchembio761
First Paragraph - Alkylation of duplex DNA in nucleosome core particles by duocarmycin SA and yatakemycin | Full Text - Alkylation of duplex DNA in nucleosome core particles by duocarmycin SA and yatakemycin | PDF (270 KB) - Alkylation of duplex DNA in nucleosome core particles by duocarmycin SA and yatakemycin | Supplementary information | Chemical compounds
See also: News and Views by Gates
Dynamic polymorphism of single actin molecules in the actin filament - pp83 - 86
Jun Kozuka, Hiroaki Yokota, Yoshiyuki Arai, Yoshiharu Ishii & Toshio Yanagida
doi:10.1038/nchembio763
First Paragraph - Dynamic polymorphism of single actin molecules in the actin filament | Full Text - Dynamic polymorphism of single actin molecules in the actin filament | PDF (1,034 KB) - Dynamic polymorphism of single actin molecules in the actin filament | Supplementary information
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Articles
Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited - pp87 - 94
Brian P English, Wei Min, Antoine M van Oijen, Kang Taek Lee, Guobin Luo, Hongye Sun, Binny J Cherayil, S C Kou & X Sunney Xie
doi:10.1038/nchembio759
Abstract - Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited | Full Text - Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited | PDF (486 KB) - Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited | Supplementary information | Chemical compounds
See also: News and Views by Walter
Allosteric inhibitors of Bcr-abl–dependent cell proliferation - pp95 - 102
Francisco J Adrián, Qiang Ding, Taebo Sim, Anastasia Velentza, Christine Sloan, Yi Liu, Guobao Zhang, Wooyoung Hur, Sheng Ding, Paul Manley, Jürgen Mestan, Doriano Fabbro & Nathanael S Gray
doi:10.1038/nchembio760
Abstract - Allosteric inhibitors of Bcr-abl-dependent cell proliferation | Full Text - Allosteric inhibitors of Bcr-abl–dependent cell proliferation | PDF (386 KB) - Allosteric inhibitors of Bcr-abl–dependent cell proliferation | Supplementary information | Chemical compounds
See also: News and Views by Ohren & Sebolt-Leopold
Microarray-based method for monitoring yeast overexpression strains reveals small-molecule targets in TOR pathway - pp103 - 109
Rebecca A Butcher, Bhupinder S Bhullar, Ethan O Perlstein, Gerald Marsischky, Joshua LaBaer & Stuart L Schreiber
doi:10.1038/nchembio762
Abstract - Microarray-based method for monitoring yeast overexpression strains reveals small-molecule targets in TOR pathway | Full Text - Microarray-based method for monitoring yeast overexpression strains reveals small-molecule targets in TOR pathway | PDF (297 KB) - Microarray-based method for monitoring yeast overexpression strains reveals small-molecule targets in TOR pathway | Supplementary information | Chemical compounds
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Top of pageCorrigendum
Corrigendum: The emerging biology of the nitrite anion - p110
Mark T Gladwin, Alan N Schechter, Daniel B Kim-Shapiro, Rakesh P Patel, Neil Hogg, Sruti Shiva, Richard O Cannon, III, Malte Kelm, David A Wink, Michael Graham Espey, Edward H Oldfield, Ryszard M Pluta, Bruce A Freeman, Jack R Lancaster, Jr, Martin Feelisch & Jon O Lundberg
doi:10.1038/nchembio0206-110
Full Text - Corrigendum: The emerging biology of the nitrite anion | PDF (52 KB) - Corrigendum: The emerging biology of the nitrite anion
