Supplementary information
From the following article
Quantitative analysis of the activation mechanism of the multicomponent growth-factor receptor Ret
Sandra Schlee, Paul Carmillo & Adrian Whitty
Nature Chemical Biology 2, 636 - 644 (2006) Published online: 1 October 2006
doi:10.1038/nchembio823
Supplementary Fig. 1
Quantification of Ret and GFR
3 expression on NB41A3-mGFR3 cells by FACS.
Supplementary Fig. 2
Contribution of ligand-dependent trafficking to the shape of the ART-dependent dose-response curves measured by KIRA ELISA.
Supplementary Fig. 3
Binding of blocking antibody GFRA3B to GFR3 as measured by flow cytometry.
Supplementary Fig. 4
Plots of experimental receptor activation data with best fits to equilibrium models 1–4.
Supplementary Fig. 5
Comparison of Ret phosphorylation quantified by western blot and KIRA ELISA.
Supplementary Methods
Description of methods and representative input file for the DynaFit modeling.
Supplementary Data
Estimated parameters with standard errors for models 1–4 in the global fitting analysis.
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