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Protein engineering

Redirecting membrane machinery

Nature Chemical Biology volume 13, pages 927–928 (2017)Cite this article

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The ability to solubilize membrane proteins while retaining their native function is a persistent challenge. Re-engineering of the membrane protein DsbB into a soluble cytoplasmic version maintained its activity and enabled recompartmentalization of the periplasmic DsbAB disulfide bond–forming system.

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Figure 1: Recompartmentalization of the periplasmic DsbA–DsbB disulfide bond-forming system.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, California, USA

    Kalistyn Burley

  2. Department of Pharmaceutical Sciences and the Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, California, USA

    Celia W Goulding

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  1. Kalistyn Burley
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  2. Celia W Goulding
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Correspondence to Celia W Goulding.

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The authors declare no competing financial interests.

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Burley, K., Goulding, C. Redirecting membrane machinery. Nat Chem Biol 13, 927–928 (2017). https://doi.org/10.1038/nchembio.2451

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