Supplementary information
From the following article
C3-symmetric peptide scaffolds are functional mimetics of trimeric CD40L
Sylvie Fournel, Sébastien Wieckowski, Weimin Sun, Nathalie Trouche, Hélène Dumortier, Alberto Bianco, Olivier Chaloin, Mohammed Habib, Jean-Christophe Peter, Pascal Schneider, Bernard Vray, René E Toes, Rienk Offringa, Cornelis J M Melief, Johan Hoebeke & Gilles Guichard
Nature Chemical Biology 1, 377 - 382 (2005) Published online: 6 November 2005
doi:10.1038/nchembio746
Supplementary Fig. 1
Binding of anti-human CD40 mAb 5C3 to CD40 does not prevent subsequent binding of 1 or hCD40L:CD8 as seen by SPR experiments.
Supplementary Fig. 2
Scanning single amino acid substitutions within the CD40 binding motif of 1.
Supplementary Fig. 3
Compound 11, a biotinylated analog of ligand 2 binds to CD40, induces lymphoma apoptosis and interacts with membrane-associated CD40 molecule.
Supplementary Fig. 4
Maturation of the murine dendritic cell line D1 by CD40L mimetics.
Supplementary Fig. 5
Preparation and characterization of CD40L mimetic 1.
Supplementary Table 1
Summary of inhibition experiments and measured kinetic values for the interaction of the different ligands with CD40.
Supplementary Methods
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