Supplementary information
From the following article
Imaging nanometer domains of 
-adrenergic receptor complexes on the surface of cardiac myocytes
Anatoli Ianoul, Donna D Grant, Yanouchka Rouleau, Mahmud Bani-Yaghoub, Linda J Johnston & John Paul Pezacki
Nature Chemical Biology 1, 196 - 202 (2005) Published online: 7 August 2005
doi:10.1038/nchembio726
Supplementary Fig. 1
Examples of analyses of numbers of secondary antibodies (Ab) per cluster based on fluorescence intensities and an antibody dilution calibration.
Supplementary Fig. 2
Fluorescence microscopy images comparing wet vs. dry H9C2 cardiac myocytes stained with DAPI and with an anti-2AR Ab.
Supplementary Fig. 3
Fluorescence microscopy images of 2AR-GFP fusion proteins overexpressed in HEK293 cells.
Supplementary Fig. 4
Analyses of two sets of colocalization data from NSOM imaging of neonatal mouse cardiac myocytes using Image J software with the Colocalization Threshold plug-in.
Supplementary Fig. 5
An example of cluster analysis performed for NSOM images.
Supplementary Table 1
Cluster densities for individual images for 2AR and 1AR in H9C2 cells and for 2AR in neonatal cardiac myocytes.
Supplementary Methods
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